目的 为更深入地研究中华芦荟活性成分的组成及化学结构。方法 采用 DEAE- Sephadex A- 2 5作为填料,通过离子交换柱层析分离。结果 从中华芦荟原始多糖中分离并提纯得到一种中性糖,经过 IR、HPL C、TGA、¹³CNMR、碱性水解及元素分析测定,证明这是一种纯度较高的、部分乙酰化的β- 1,4- D-聚吡喃甘露糖,其中糖链中甘露糖与乙酰基的物质的量之比为 1.6 4∶ 1。结论 该多糖为首次分得。

Object To carry out a deeper study on the active component and the structure of Aloe vera L. var chinensis (Haw) Berg Methods The polysaccharide was isolated from the extract of the fresh leaf pulp of A. vera var chinensis by means of DEAE Sephadex A 25 ion exchange column chromatography and identified, through HPLC, IR, TGA, ¹³CNMR and elementary analysis Results Alkaline hydrolysis proved that it is a neutral, linear polymer of a partially acetylated β- 1, 4-D mannopyranose of high purity The molar ratio of D mannose to the acetyl group in the polysaccharide was 1 64∶1 Conclusion This is the first report to uncover the presence of such polysaccharide in A vera var chinensis