[关键词]
[摘要]
目的 研究槐胺碱 (SA)对移植排斥反应的影响并探讨其发生机制。方法 以同种皮肤移植大鼠作为动物模型,免疫荧光浊测定 T淋巴细胞及其亚群数量 ;MTT比色法测定 T淋巴细胞增殖反应。结果 SA减少 CD3、CD4细胞数,降低 CD4/ CD8,抑制 T淋巴细胞的增殖,延长移植物存活时间。结论 SA对移植排斥反应有抑制作用,此作用与其减少 T淋巴细胞数量并抑制 T淋巴细胞功能有关
[Key word]
[Abstract]
Object In order to obtain further evidence of ther apeutic efficacy of sophoramine (SA)*, its effect on transplantation rejection was studied with simultaneous exploration of its mechanism of action. Methods Number of T lymphocyte and its sub-sets of skin allografted ra t model were determined by immunofluorescence technique, and proliferation of T lymphocyte was assessed by thiazolyl blue (MTT) assay. Results SA reduced the CD3 and C4 cell counts, lowered CD4/C8 ratio, inhibited T lymphocyte proliferation and prolonged the survival time of skin allografted rat. Conclusion SA could inhibit transplantation rejection by inhibiting the function of T lymphocyte with simultaneous reduction of lymphocy te count.
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[基金项目]
辽宁省教委资助