目的 研究甘草酸粗品的提取工艺。方法 采用正交试验法,以甘草酸粗品收率及 2 5 1nm处紫外吸光度值为指标,考察加水量 (A)、煎煮时间 (B)和酸沉 p H值 (C) 3个因素,并采用单因素试验考察加水量及药材粉碎度对甘草酸粗品收率的影响。结果 优化的甘草提取工艺条件为 A₁ B₂ C₁ 。加水量≥ 2 4倍甘草能得到较完全提取。不同粉碎度提取所得的甘草酸粗品收率依次为 :最粗粉≌粗粉 >细粉 >饮片。结论 加水回流 2次 (15倍,1.2 5 h,12倍,1h),酸沉为 p H 1。加水量≥ 2 4倍基本能提取完全。提取时以最粗粉或粗粉作为原料,较饮片及细粉好

Object To optimize the extraction p rocess of the root of Glycyrrhiza uralensis Fisch. in order to improve the yield of crude glycyrrhizic acid for the preparation of new drug dos age forms of the well known TCM recipe SINITANG DECOCTION. Methods The study was carried out through orthogonal experimental design, using UV absorbance of glycyrrhizic acid at 251 nm as the guide. 3 influencial factors, amount of water used for the extraction (A), time for extraction (B) and pH valu e for the precipitation of the acid (C) were studied. In addition, a single fac tor test between the amount of water used and the fineness of the herbal materia l was also studied. Results The optimal conditions for the ext raction were A1B2C1. Decoction with 24 or more times of water can achieve complete extraction. The fineness of the herbal material that effects the yield were in the increasing order of most coarse ≌ coarse ＞ fine powder ＞ sliced h e rb. Conclusion Extract twice, first with 15 times of water for 1.25 h and then with 12 times of water for 1 h, and precipitate the acid at pH 1 will give the best yield. The most suitable fineness of the raw herbal mater ial were most coarse to coarse. They give better yield than fine powder or sliced herb.

“211工程”重点建设项目课题,项目号:98176;广东省高教厅资助