[关键词]
[摘要]
目的建立毛细管电泳法(CE)测定贝母中浙贝乙素、西贝素、西贝苷等生物碱的含量测定方法。方法以毛细管区带电泳模式,用内标法分析了9种贝母药材中上述生物碱的含量。结果3种生物碱分离完全,并有较好的回收率和重现性。结论本法快速、简便、高效,为贝母的质量控制和评价提供了可靠依据
[Key word]
[Abstract]
Object To develop a method for the quantitative anal ysis of peiminine, imperialine and imperialine-3β-D-glucosid e i n Fritillaria L. by capillary electrophoresis. Methods 9 species of bulbus Fritillaria L. were analysed with in ter nal standard by the mode of capillary zone electrophoresis. Results The 3 alkaloids were separated completely with the method which showed good recovery and reproducibility. Conclusion The method was prove d to be quick, simple, and efficient, and provided a reliable basis for the qual ity control and evaluation of this plant medicine.
[中图分类号]
[基金项目]
上海市教委重点学科课题资助