为了鉴别金线莲的品种, 探索同属植物之间的亲缘关系及同种植物不同产地的遗传变异, 并制定正品金线莲的DNA指纹图谱, 采用随机扩增多态DNA(RAPD)技术对兰科开唇兰属植物花叶开唇兰Anoectochilus roxburghii(Wal l.).Lindl.、台湾开唇兰A.formosanus Hay.进行了鉴定, 结果选择的7个引物对3个品种共扩增出98个位点, 其中3个引物为高特异性引物.结论RAPD术不仅能鉴别种间差异, 而且能揭示同种不同产地植物的遗传变异.

In order to explore the consanguinity of plants of genus Anoectochilus Bl. (Orchidaceae), and to dis tinguish Anoectochilus roxburghii (Wall.) Lindl. from two diffe r ent localities, Fujian and Guangxi Province, and A. formosanys Hay as to their genetic variance, DNA fingerprintings were analysed with the ai d o f RAPD. Results showed that 7 primers amplified a total of 98 sites for the 3 s pecies. 3 of the 7 primers were highly specific for genuine A. roxburgh i i. Thus, the 3 species could be distinguished according to the bonding pattern of their amplified DNA on agarose gel. It was concluded that the method may be of value for the authentication of different species and same species of Anoectochilus Bl. from different localities

厦门市卫生局医学科学技术进步基金科研资助项目