目的:在考察淫羊藿苷 (icariin)于人工胃液中稳定性的基础上, 研究了肠道内细菌对淫羊藿苷的代谢作用。方法:于人工胃液或肠内菌培养液中, 加入淫羊藿苷温孵培养一定时间后, 以薄板层析、高效液相色谱仪和电喷雾质谱仪, 对培养物成分做定性分析检查。结果:淫羊藿苷在人工胃液中有较高的稳定性。离体培养人肠道内细菌可代谢淫羊藿苷, 且其主要代谢产物为淫羊藿苷的苷元 (icaritin)及其苷元的异戊烯基位置异构体。在大鼠整体实验中, 从粪便和尿液中均检出一主要代谢产物, 并初步确定此代谢产物为宝藿苷Ⅰ(baoliuosideⅠ)。结论:在离体条件下, 淫羊藿苷可被人肠内菌代谢, 主要代谢产物为淫羊藿苷的苷元。大鼠灌服淫羊藿苷后, 吸收入血的主要代谢物为宝藿苷Ⅰ。

The stability of icariin in artificial gastric juice and the transformation of icariin in intestine were studied. Icariin was incubated with artificial gastric juice or intestinal flora for a certain period of time, and its metabolite examined by TLC, HPLC and ESI/MS. results showed that icariin was stable in artificial gastric juice, but was metabolized to icaritin by human intestinal bacteria in vitro. A main metabolite, baohuosideⅠ. was found in urine and feces of rat after ig administration of 200 mg/kg icariin. These results showed that icariin can be metabolized in vitro by human intestinal bacteria to icaritin and as baohuoside after oral administration.

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