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[摘要]
探讨确定西洋参种子的贮存习性,进而为种质资源长期保存和生产实践提供理论依据及方法。本实验用硅胶干燥西洋参种子,使其含水量由风干时的5.9%降至3.4%和1.7%。3种含水量的种子密封后置室温、0~5℃、-18℃和-196℃(液态氮)下保存。干燥后保存前用 TTC法测定种子生活力。保存近半年后,用等电聚焦分析酯酶同工酶,检测种子裂口率、种子根、芽长度和种子田间出苗率等指标,综合评价种子生活力、活力水平。结果显示:干燥脱水后西洋参种子生活力仍然很高,TTC染色率均为100%;在4种不同温度保存近半年后,种子裂口率、田间出苗率均在72 %和61%以上,处理间方差分析均未达显著水平(F
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[Abstract]
Seeds of Panax quinquefolius L with original moisture content of59%, together with that desiccated over silicagel to moisture contents of34% and17%, were stored at ambient temperature,5 ℃,18 ℃ and196 ℃(liquidnitrogen)for6 months Influences of desiccation and storage on seed viability were tested by TTC staining, before and after desiccation, and their vigour were evaluated on tests of seed esterase isozyme isoelectric focusing(IEF), and field test on their rate of splitting and emergence,length of bud and root Results showed that there wasno effects on seed viability by desiccation to34% and17% of moisture content, as the TTC staining rates were100% for all seeds tested immediately after desiccationno significant differences in seed viability and vigour were detected among seeds stored at defferent temperature as there areno significant difference in vairance analysis for seed split rate and seed emergency rate.
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