采用反相高效液相色谱蒸发激光散射检测器测定三七中人参皂苷 Rg1 的含量。色谱柱为 CL C-ODS（6.0mm×150mm），流动相为乙腈-水（30：70），蒸发激光散射检测器。定量方法简单，准确。回收率为100.50%，RSD为1.82 %。并用紫外分光光度法测定了三七中总皂苷的含量，结果和 HPLC法基本一致，说明该方法定量准确，回收率为101.50%，RSD为1.44 %。结论认为两方法均可以控制三七的质量。

A reverse phase HPLC ELSD method for the determination ofginsenoside Rg1 in the root of Panax pseudoginseng var.notoginseng（Burkill）Hoo et Tseng was reported. Chromatographic conditions: Shim pack CLC ODS column（60mm×150mm); acetonitrile water（30:70）as the mobile phase; Shimadzu LC6A with SEDEX55 ELSD detector. The method was found to be simple and accurate with recovery rate of10050% and RSD=182%. The established UV spectrophotometric determination of total saponins in P. pseudoginseng var.notoginseng was also tried andgave an accurate result coincidental with that of the HPLC results. The recovery rate was10150%, and RSD=144%. It seemed that both methods can be used reliably for the quality control of P. pseudoginseng var.notoginseng.