为探讨葛属植物间的亲缘关系,并为分类和鉴定提供依据,采用RAPD技术对葛属6种植物进行分析,利用RAPD(Version4.0)及PHYL IP(Version3.5C)软件包进行数据处理,UPGMA法聚类分析得到系统树。结果发现,峨嵋葛与野葛间的遗传距离小,应归并为野葛,野葛与粉葛、山葛间的遗传距离大,粉葛、山葛应独立成种。DNA指纹图谱可用于葛属植物间的鉴别

To explore the affinity relation of Pueraria DC plants and to provide evidence for their classification and authentication, RAPD analysis was performed on 6 species of Pueraria DC found in China.The DNA fingerprints was analysed with the aid of RAPD (Version 4.0) and PHYLIP (Version 3.5 C).The results showed that the genetic distance of P.omeiesis was close to P.lobata, while that between P.lobata and P.thomsonii and P.montana were longer in evolution.Therefore, it could be concluded that P.thomsonii and P.montana were not the variants of P.lobata, but was another independent species.RAPD amplification fingerprinting can also be used to identify Pruearia DC plants.