[关键词]
[摘要]
体外用细胞生长曲线法、MTT试验、蛋白质含量测定法及形态学观察研究了白首乌甾体总苷(CG)的抗肿瘤作用。结果表明:CG对Hce-8693、PC3、Hela、PAA4种实体瘤细胞均有较强的体外细胞毒作用,且呈浓度依赖性。CG作用于Hela细胞4d,形态观察表现为细胞膜皱缩、细胞核固缩、浓聚
[Key word]
[Abstract]
In vitro cytotoxicity of total steroidal glycoside (CG) from the root of Cynanchum auriculatum Royle ex Wight was studied on Hce 8693, PC 3, Hela and PAA solid tumor cells by determining cell growth curve, MTT test, protein content assay and morphological observation. The results showed that CG exhibited potent cytotoxic effect on all four solid tumor cells lines in a concentration dependent manner. After 4 d of treatment with CG, Hela cells proliferation distinctly declined with dramatic morphologic alteration, including some cell membrane shrinkage and nuclei pyknosis.
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[基金项目]
浙江省自然科学基金资助!No396484