[关键词]
[摘要]
三裂叶野葛的叶柄和茎段外植体在含有6-BA1.0mg/L和NAA0.1mg/L的MS培养基上产生愈伤组织,愈伤诱导率分别为100%和94%,并进一步分化出芽,芽再生率分别为100%和33.3%。而在添加6-BA3.0mg/L和NAA1.0mg/L的MS培养基中叶柄和茎段外植体的愈伤诱导率分别为100%和64.3%,芽再生率分别为57.1%和21.4%。芽长成嫩枝后转移至添加0.1mg/LNAA的MS培养基后分化出根,形成小植株。再生植株的移栽成活率达90%。
[Key word]
[Abstract]
The callus induction rates on MS medium containing 6-BA 1.0 mg/L + NAA 0.1 mg/L were 100% for petiole explant and 94% for stem explant. Callus could differentiate buds on the same medium, shoot regeneration rates were 100% and 33.3% respectively. While cultured in the medium supplemented with 6-BA 3.0 mg/L and NAA 1.0 mg/L, the callus induction rates were 100% and 64 3% respectively, and the shoot regeneration rates were 57.1% for petiole explant and 21.4% for stems. When the shoots were transferred onto MS medium containing 0.1 mg/L NAA, they differentiated into roots and formed regenerated plants. The survival rate was 90%.
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[基金项目]
广州市科委资助项目 (1999-J-0 0 70 1)