用RAPD方法分析了苍术不同居群间的亲缘关系。选取10个10碱基的随机引物对苍术的10个居群共83个个体进行扩增,获得100个 DNA片断。用RAPD istance软件计算了不同居群间的Jaccard遗传距离,得距离矩阵,分别用单联法、全联法、类平均法作聚类图,结果表明,该10个居群可划分为3个类群:即类群1,大别山类群(Ta);类群2,南苍术类群(Wu、L u、Hu、Xu、Ca、L i);类群 3,北苍术类群(Tt、Da、L ao)。在这3个类群中,南、北苍术之间的遗传距离相对较近,大别山类群与南、北苍术之间的距离则较远。根据本试验的结果及前人关于苍术精油成分差异的聚类结果,作者认为大别山类群比较特殊。

The relationships between the populations of Atractylodes lancea (Thunb.)DC.were analyzed with the method of rand om amplified polymorphic DNA(RAPD).Choosing 10 primers of 10-base oligonucleot ide to amplify the DNA of A.lancea which have 10 populations an d contain 83 individuals, 100 fragments were obtained. Using RAPD distance soft ware, the Jaccard's distance was caculated and listed in the form of distance's matrix, and from which the dendrograms were drawn using the methods of nearest n eighbor clustering, furthermost neighbor clustering and UPGMA separately.The re sults showed that the 10 populations could be divided into 3 clusters, namely cl uster 1 ［Dabieshan (Ta)］， cluster 2 ［South A.lancea (Wu, Lu , Hu, Xu, Ca, Li)］ and cluster 3 ［North A.lancea (Tt, Da, Lao )］. Furthermore, among the three clusters the distance between cluster 2 and c luster 3 was obviously nearer than the distance between cluster 2 and cluster 1 or the distance between cluster 3 and cluster 1. According to this result and t hat reported elsewhere by other workers concerning the essential oil components ， it was suggested that cluster 1 sho uld be listed alone from the south A.lancea.