[关键词]
[摘要]
为观察灵芝多糖(GLB7)体外对小鼠腹腔巨噬细胞(MΦ)蛋白激酶A(PKA)活性的影响,采用反相离子对高效液相色谱法测定MΦ中RKA活力。结果表明GLB7(40mg-L)能引起小鼠腹腔MΦ中PKA活性明显升高,10min达峰值,30min恢复到基础水平。提示灵芝多糖的免疫增强作用与其增强MΦ中PKA活性有关。
[Key word]
[Abstract]
To investigate the effects of Ganoderma lucidum polysaccharide (GLB7) on protein kinase A (PKA) activity of murine peritoneal macrophages. A new ionpair RP-HPLC method was used to determine the activty of PKA in cells. The results showed that GLB7 (40 mg-L) activated the PKA of murine peritoneal macrophages, to a peak value within 10 min, and then recovered to its basic level after 30 min. This result indicated that GLB7 may act as an immunopoteniator of PKA in murine peritoneal macrophages.
[中图分类号]
[基金项目]
国家自然科学基金资助项目No-39400165