[关键词]
[摘要]
建立测定密骨胶囊中淫羊藿苷含量的方法。样品用甲醇超声提取30min,在C18柱上以甲醇-水-醋酸(45∶50∶5)为流动相,在波长270nm处检测。淫羊藿苷含量测定的线性范围为0.0509~0.8144μg,线性回归方程为Y=1479.437+455051.213X,r=0.9999,平均回收率为98.95%,RSD为2.44%。该法简便、准确、重现性好,可作为密骨胶囊质量控制的方法。
[Key word]
[Abstract]
To develop a method for the determination of the contents of icariin in Migu Capsule, a compound Chinese herbal preparation containing Epimedium koreanum Nakai for the treatment of osteoporosis, by RP-HPLC. The sample was shaken in an ultrasonic bath for 30 min. The contents were determined by HPLC, with column C18 as stationary phase, methanol-water-acetic acid (45∶50∶5) as mobile phase and UV detector at 270 nm. The content of icariin had a good linearity in the range of 0.050 9~0.811 44 μg, Y=1 479.437+455 051.213 X, r=0.999 9. The average recovery is 98.95% and RSD is 2.44%(n=5). The method is rapid, reliable and simple, and can be used for the quality control of Migu Capsule.
[中图分类号]
[基金项目]
国家“九·五”攻关资助项目(No.96-906-09-05)