采用 HPLC法同时测定车前草中熊果酸、齐墩果酸的含量。选用 C₁8 ODS色谱柱，流动相：甲醇－水（88：12），检测波长：220μm，流速：0.6mL/min，柱温：25℃。熊果酸和齐墩果酸分别在0.4042∼2.020μｇ（r=0.9984）和0.410∼2.050μｇ（r=0.9997）范围内呈线性；熊果酸、齐墩果酸的平均回收率分别为98.2%（RSD=1.74%）和97.8%（RSD=2.24%）。该法准确、快速、灵敏，重现性好。

The contents of ursolic acid and oleanolic acid in Plantago asiatica L.was determined by HPLC. The HPLC system consisted of C, 8-ODS, mobile phase of methanol-water (96,4) mixture, with detection at 220 nm, flow rate 0. 6 mL/min and column temperature 25 C.The linear ranges of determination of ursolic acid and oleanolic acid were 0.404-2. 020 pg, r=0. 998 4 and 0. 410-2. 050 μg, r=0. 999 7, respectively. The mean recovery of 98. 2%, RSD 1.74%, for ursolic acid and 97.8%, RSD 2.24% for oleanolic acid is obtained. The method is sensitive, rapid and accurate with good reproducibility.

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