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[摘要]
用HPLC法测定蒲公英中咖啡酸和绿原酸的含量。采用HP Hypersilc18色谱柱,200 mm× 2.1mm,5µm,流动相:甲醇-磷酸盐缓冲溶液(pH=4.2)23:77,检测波长:323 nm,柱温:40℃,流速:1.0 mL/min。该法咖啡酸平均回收率99.80%,RSD 2.14%;绿原酸平均回收率99.54%,RSD 2.69%。本法稳定可靠,为药典建立蒲公英药材的质量标准提供了依据。
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[Abstract]
The establishment of a quality standard by HPLC for caffeic acid and chlorogenic acid in dandelions, Tarazacum Weber, was reported. The sample was extracted with methanol under ultrasonic management for 30 min. Chromatographic conditions:YMS-ODS C, e column, 200 mm × 2.1 mm, 5 μm, a mobile phase consisted of methanol and potassium phosphate (23:77, pH 4.2), a flow rate of 1. 0 mL/min;a detecting wavelength at 323 nm and the column temperature at 400C.The mean recovery rate of caffeic acid was 99. 80%, RSD 2.14 % . The mean recovery rate of chlorogenic acid was 99. 54%, RSD 2. 69 %. The method was very stable, accurate, and can be used as a quality standard for dandelions.
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