采用原位杂交和RT-PCR方法，观察消瘀片治疗16周动脉粥样硬化免后对血管壁组织ET-1mRNA和NOSmRNA表达的影响。结果显示，粥样硬化组的ET-1mRNA和iNOSmRNA表达较多，而cNOSmRNA表达较少。使用消瘀片后，动脉壁组织中的 ET-1mRNA表达明显减少，提示血浆和血管壁组织中的 ET-1减少是由于 ET-1mRNA转录水平降低所致。消瘀片对血管壁组织中iNOSmRNA和cNOSmRNA表达影响是不同的，它可降低iNOSmRNA表达，增加cNOSmRNA表达。表明消瘀片可通过调节 NOS不同亚型基因的表达而消减动脉粥样硬化病变的程度.

Techniques of in situ hybridization and RT-PCR as well as image pattern analysis were used in this study. The results indicated that ET-1 mRNA and iN()SmRNA expression in atherosclerotic model group were obviously increased，while cNOS, mRNA expression was decreased. After treatment with Xiaoyu tablet，a drug containing extracts of .Salvia miltiorrhiza Bunge and C'rutaegus pinnatfida Bunge for the re-moval of blood stasis，for 16 weeks，EZ、一1mRNA expression of vessel wall was reduced，suggesting that re-duction of ET-1 in plasma and vessel wall was due to inhibition of ET-1 mRNA transcription. Effects of Xi-aoyu tablets on iNOSmRNA and cNOSmRNA expression were very different， the drug might reduce iNOS mRNA expression and enhance cNOSmRNA expression, indicating that Xiaoyu tablet might abate the atherosclerotic degree through regulating the expression of NOS subgroup genes.

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