[关键词]
[摘要]
吸附和扩散特性是进行色谱过程的基础, 本文测定了紫杉醇及其类似物三尖杉磷碱在多孔硅胶上的吸附及扩散特性, 结果表明:紫杉醇和三尖杉磷碱在硅胶上的吸附不符合Langmuir单分子层吸附理论, 但在低浓度下可用溶剂调整型Langmuir模型描述。扩散研究表明;紫杉醇和三尖杉磷碱在硅胶上吸附较弱, 表面扩散可以忽略。相同条件下, 紫杉醇的吸附量受强溶剂影响较大, 强溶剂组成的增加会造成紫杉醇吸附量很快下降.这和在正相色谱中紫杉醇先于三尖杉磷碱流出色谱柱的事实是相符的。
[Key word]
[Abstract]
Adsorption and diffusivity behavior of paclitaxel and cephalomanine on porous gel during chromatographic separation were studied. Results showed that their adsorption did not follow Langmuir model, but at low concentrations of the solute, the solvent modulated Langmuir model could be followed and gave satisfactory results. As to their diffusivity, the rate on the surface of the absorbent could be negelected due to the fact that both paclitaxel and cephalomanine were very little adsorbed. Under the same condition, the increase of strong solvent resulted in a greater influence on the adsorption of paclitaxel by decreasing its adsorption rapidly as compared with cephalomanine.
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[基金项目]
国家教委“跨世纪优秀人才培养计划”和教委优秀青年教师基金