采用高效薄层预制板,用二次展开的方法分离测定了毛白杨叶中的特里杨苷等三种酚苷类化合物。以二氯甲烷-乙酸乙酯-甲醇-水(2:1.5:0.5:0.05)为展开剂,α萘酚-硫酸试液为显色剂,用 CS-930薄层扫描仪,λ=520 nm波长处线性扫描。方法简便、快速、准确。

A double development thin layer chromatographic method was established to separate and determine the amount of tremulacin, treulodin and salicin in the leaves of Populus tomentosa Carr. by HPTLC plate. The plates were developed twice in a solvent system of dichloromethane-ethyl acetate-methanol-water (2∶1.5∶0.5∶0.05) and color development with α-naphthol-sulfonic acid-ethanol solution. The detection wavelength was 520 nm. The results showed that this method was simple, rapid and accurate.