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[摘要]
以自然衰老小鼠为实验对象,采用邻苯三酚自氧化法测定SOD活性、紫外分光光度法测定CAT活性、愈创木酚法测定POD活性、DTNB直接比色法测定GSH-Px活性,研究了鲤鱼精巢DNA对老龄动物体内抗氧化酶活性的影响。结果表明,鲤鱼精巢DNA可明显提高自然衰老小鼠 体内抗氧化酶的活性。
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[Abstract]
Effects of carp spermary DNA on various oxidoreductase activities in naturelly senile mice were investigated. The activities of superoxide dismutase (SOD),catalase (CAT),peroxidase (POD) and glutathione peroxidase (GSH-Px) were measured by means of pyrogallol auto-oxidation, ultraviolet spectrometry, guaiacol oxidation and DTNB (5,5‘-dithiobis-2-nitrobenzoic acid) direct colorimetry, respectively.The results showed that DNA from carp spermary could enhance the activities of these enzymes in naturally senile mice.
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