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[摘要]
应用反相 HPLC法测定芦荟甘(aloin)的含量。用 SUPELCO LC-18(250 min×4. 6 mm,5um)层析柱,流动相:乙腈-0.01 mol/L三氟醋酸(22:78),流速:1mL/min,检测波长:359 nm,柱温:室温,用外标法测定。芦荟苷的平均回收率为 98.37%,其检测线性范围为 50~250ug/mL,回归方程:Y=0.269+54.723 X,r=0. 9999.本法灵敏、简便,重现性好。
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[Abstract]
A RP-I-IPLC method was developed for the determination of aloin in Aloe barbadensis Mill..and A. ferox Mill. An ODS column was used with the mobile phase of acetonitrile-0. 01mol/L trifluoracetic acid (22:78) at a flow rate of 1 mL/min. Samples were detected at 359 nm and at room temperature. External standard was used and the calibration curve was linear over the range of 50~250ug/mL(r=0.9999). The extraction recovery rate was 98. 37%.This method is sensitive, simple and well reproducible.
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