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[摘要]
采用sc角叉菜胶(Car)25mg/kg复制大鼠背部气囊滑膜炎模型。白细胞计数、蛋白含量分别用试管稀释法和Lowry法测定。酶联免疫吸附分析法、Rivanol法和Fura-2荧光分析法分别用于测定灌洗液中TNF、NO含量以及中性粒细胞胞内游离钙(Neu-[Ca2+]i)水平的变化。结果显示:三七总皂苷(PnS)60、120、240mg/kg 3个剂量组均能一定程度地降低灌洗液中白细胞数、蛋白含量、TNF及 NO含量;与此同时,抑制 Neu-[Ca2+]i水平的升高,其抑制率分别为: 9.2%、34.3%及39.2%.提示PnS具有良好抗炎活性,其机制与抑制灌洗液中炎性细胞因子TNF及NO水平的升高有密切关系,而抑制Neu-[Ca2+]i水平的升高则是PnS抑制炎性细胞因子含量升高的重要分子机制之一。
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[Abstract]
Rat air-pouch acute imflammatory model was prepared by sc injection of carrageenan (Car,25 mg/kg). The neutrophil (Neu) count and protein content in exudate were measured by test tube diluting method and Lowry method. Enzyme linked immunosorbent assay and Rivanol assay were applied respectively to investigate effects of saponins of Panax pseudo-ginseng var. notoginseng (Burk.) Hoo et Tseng (PnS) on tumor necrosis factor (TNF) and nitric oxide (NO) content in the perfusate. Fura-2 fluorescence technique was used to determine the intracellular free calcium concentration in Neutrophils (Neu-[Ca2+]i). The results showed that PnS 60, 120 and 240 mg/kg ip reduced Neu counts, protein content, TNF and NO contents in a dose-dependent manner; lowered the level of Neu-[Ca2+]i with inhibitory rates of 9.2%, 34.3%, and 39.2%, respectively. It was concluded that PnS has an obvious antiinflammatory effect and its mechanisms of action are related to the reducton of TNF and NO contents, and inhibition of Neu-[Ca2+]ilevel.
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