应用毛细管区带电泳法,对伸筋丹胶囊中的士的宁和马钱子碱成分进行了分离研究.采用pH=6.0的0.2 mol/L.tris-磷酸溶液作为缓冲液;运行电压20 kV;检测波长为260nm,被测成 分可得到较好分离.用内标法测定其含量,结果表明,在 0.02～0.15 mg/mL范围内,士的宁和马 钱子碱均与内标峰面积之比呈良好线性关系,平均回收率分别为95.1%(RSD=2.8%)和96.4% (RSD=3.0%).

A capillary zone electrophoresis method was established for the determination of strychnine and brucine in Shenjindan capsule,a preparation composed of different TCM including Semen Strychni and dried earthworm for relieving rigidity of muscles by promoting blood circulation to remove blood stasis.0.2mol/L tris-H3PO4(PH=6.0)was used as running buffer, running voltage was 20KV, and UV absorbance detection was set at 260 um, This method can give a good separation for the determination of strychnine and brucine.By using internal standard the calibration curve showed good linearity for strychnine and brucine,average recovery was 95.1% for strychnine and 96.4% for burcine.