[关键词]
[摘要]
怀山药带节茎段在 MS+6-BA1-2mg/L+NAA 0.1-1mg/L的培养基上培养,均能直接形成多芽体,均芽数为3-5.将多芬体转入MS或MS+KT 2mg/L+NAA 0.02mg/L+PP3 33 0.1mg/L的培养基上均能诱导生根,形成再生植株.但后者形成的苗生长健壮,叶色浓绿,有较强的生活力.
[Key word]
[Abstract]
Dioscorea opposita Thunb.was micropropagated from nodal culture.This species produced 3 ~5 buds per node when cultured on modified MS Medium supplemented with 6-BA 1-2mg/L and NAA 0.1-1 mg/L.Root could be induced when these multibuds were transplanted onto MS basic Medium or MS basic Medium supplemented with KT 2 mg/L,PP333 0.1 mg/L and NAA 0.02 mg/L.The regenerated plantlets treated with PP333,grew robustly and developed deep green leaves with vigour.
[中图分类号]
[基金项目]
河南省自然科学基金;省科技攻关项目