RP-HPLC法对黄芪毛状根中黄芪甲苷进行定量分析,在实验条件下,黄芪甲苷标准品在 10.64~53.20μg范围内与峰面积呈良好的线性关系;平均加样回收率为92.66%;精密度试验相 对标准偏差为4.20%和2.40%;测得黄芪毛状根中黄芪甲苷的含量为0.14%(干重)。

An accurate HPLC method for the determination of astragaloside N in the hairy root cultures of Astragalus membranaceus (Fisch.)Bunge was developed. Calibration graphs was rectilinear between 10. 64μg and 53. 20μg under the experimental conditions. The recovery was 92. 66% The relative standard deviations (RSDs) of measurement precision test were 4. 20% and 2. 40% The astragaloside N content measured in the haiy root cultures was 0. 14% (dry weight).

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