采用结扎大鼠双侧颈总动脉合并低血压后再恢复供血造成大鼠脑缺血再灌注模型,运用 斑点杂交技术,观察大鼠全脑缺血再灌注中海马c-fos mRNA表达的动态变化,并观察小檗碱 (Ber)的作用.结果表明,大鼠全脑缺血30 min后再灌注10~180min,海马c-fos mRNA出现一过 性高表达,1h达高峰,为假手术组的5.1倍.Ber20mg/(kg·d)×5dip显著抑制脑缺血诱导的 c-fos高表达,且明显升高SOD及GSH-Px活力,降低MDA含量.这表明,脑缺血过程中c-fos原 癌基因呈现一过性高表达,Ber的抗氧自由基及降低c-fos mRNA水平的作用可能是其抗脑缺血 作用机制之一

Dot blotting assay was employed in this study to observe dynamic changes of c-fos mRNA expression during reperfusion after cerebral ischemia in rats, and effect of berberine (BER) on c-fos expression induced by global cerebral ischemia was studied. The results showed that c-fos gene expression was temporally increased in global cerebral ischemia by 5.1 times, as compared with sham-operated group. However, the changes of c-fos gene expression could be inhibited dramatically by BER 20 mg/(kg.d)×5 d ip. The same dosage of BER significantly increased activities of SOD and GSH-Px and reduced content of MDA of brain tissues. The results suggested that the inhibitory actions of BER on c-fos expression induced by global cerebral ischemia may be related to its protective action against brain ischemia.

江苏省科委资助!BS94021