采用小鼠离体肝灌流术在Krebs－Henseleit（K－H）灌流液中加入柴胡注射液，观察柴胡对肝缺氧（1h）复氧（1h）的影响。结果表明：柴胡组与缺氧组相比，光镜电镜下肝组织损伤较轻，且肝组织乳酸脱氢酶（LDH）漏出量显著减少，脏/体比、还原型谷胱甘肽（GSH）及黄嘌呤氧化酶（XOD）含量有显著性差异，丙二醇（MDA）生成延迟。揭示柴胡对小鼠离体灌流肝缺氧复氧损伤有明显拮抗作用，其机制与抑制氧自由基形成有关。

By using isolated mouse liver perfusion model，the protective effect of Chaihu injection on poxia-reoxygenation liver injury was studied. The activity of lactate dehydrogenase (LDH) in perfusion hypoxia-reoxygenation liver injury was studied.the content of glutathione(GSH) and maloialdehyde(MDA) in liver homogenate were investigated at different time courses. Liver pathology by light microscopy and electronic microscopy were observed after perfusion. The result implied that Chaihu injection has a significant protective effect on mouse liver injury induced by hypoxia-reoxygenation perfusion. The mechanism of action may be related to its inhibitory effect on the formation of free radicals.