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[摘要]
应用发根农杆菌(Agrobacteriiumrhizogens)感染决明无菌苗建立了决明毛状根培养体系。分析了决明毛状根中的甾醇类化学成分并初步探讨了它们的生物合成途径。用薄层色谱(TLC)、气相色谱(GLC)、高效液相色谱(HPLC)和质谱(MS)等方法从毛状根中分离鉴定了12个甾醇类成分。根据研究结果提出了其中主要甾醇类成分的生物合成途径。
[Key word]
[Abstract]
Hairy root cultures of Cassia obtusifolia induced by Agribacterum rhizogens were established to investigate the composition and biosynthesis of phytosterols.Effects of rare earth element Eu on the growth of hairy root were also investigated.Twelve different sterols were isolated from the hairy roots.They were identified by chromatographic (TLC, GLC and HPLC) and spectral methods (MS).The biosynthetic pathways leading to the production of major sterols were proposed based on the results of this study.
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