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[摘要]
从东北茜草提取得到的粗多糖经离子交换、DEAE-纤维素和SephadexG-50柱层析得到多糖QA2。QA2经HPLC的Lonpak S803柱层析为单一对称峰,证明为均一多糖,并测得其分子量为5000。TLC和GC分析表明QA2由Rha、Ara、Xyl、Man、Glc和Gal组成,其单糖摩尔比为1.0:2.5:1.4:22.8:277:15.3。红外光谱分析含β型糖甙键。药理实验结果表明QA2具有较强的清除自由基的作用,清除率为94.59%。
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[Abstract]
Polysaccharide QA2 was isolated and purified by decocting with water,followed by alcohol precipitation and DEAE-cellulose,gel filtration chromatography(Sephadex G-50).Its homogeneity was proved by HPLC on Ionpak S803 column,The average MW was 5000.According to TLC and GC analysis,QA2 was composed of rhamnose,arabinose,xylose,mannose,glucose and galactose in molar ratio of 1.0,2.5,-4,22.8:27.7:15.3.IR spectrum indieated that it has a β configuration.Preliminary pharmacological tests showed that QA2 has strong scavenging effect on free radicals of hydrogen in the free radical OH generating system.The scavenging ratio is 94.5%.
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