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[摘要]
采用HPLC定量测定天然胡萝卜素胶丸中β-胡萝卜素的含量。该法用硅胶预柱对样品进行前处理,以ODS柱为分析柱,以甲醇-乙腈(90:10)(pH3.0)为流动相,检测波长为450nm。实验表明,该方法线性良好,平均回收率为99.2%
[Key word]
[Abstract]
An HPLC method was used for the quantitative determination of β- carotene in cartene capsules. The sample was pretreated with a silica gel column,and then determined on a ODS natural column, with a mobile phase of methanol-acetonitrile (90,10) (pH 3. 0),and detected at 450nm. The linearity was very good. The average recovery was 99. 2%.
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