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[摘要]
红松果多糖经酸水解得到活性成分木脂素,用GF254薄层板,经二氯甲烷-乙醚混合溶媒展开,斑点洗脱后于230um波长测定吸收值的TLC-UV法,木脂素在2~70gμg/ml浓度范围内与吸收值呈线性。回收率在100.2%~101.4%,RSD为6.5%。
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[Abstract]
A TLC-UV method for the determination of lignin in polysaccharides of cone of Pinus koraiensis was reported. Active lignin was obtained by acid hydrolysis,and developed on GF-254 TLC-Plate by mixed Solventof dich1oro methane-ethylether. UV absorbance of the eluate was determined at 23Onm. The concentration oflignin was linearly related to absorbance within the range of 2~70pg/ml. The recovery was 100. 2% ~ 101.4% and RSD was 6. 5 %.
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