[关键词]
[摘要]
运用DEAE-纤维素柱层析和超速离心技术,从黔产葫芦科植物苦瓜MoinordicacharantiaL.果实的压榨汁中,分得一植物蛋白质,经化学和凝胶电泳鉴定,其分子量为34000,等电点为pH8.4,由17种氨基酸组成,N-末端氨基酸为亮氨酸。动物实验结果表明,该蛋白质能抑制雄鼠精子发育,具有显著的抗生育活性,有效率达88%以上。
[Key word]
[Abstract]
A protein was isolated by DEAE-cellulose Chromatography and ultracentrifuge from the fruits of Momordica charantid L., and identified by chemical and polyacrylamide gel electrophoresis methods. The molecular weight was 34 000; isoelectric point pH 8.4,N-end amino acid was leucine.It was composed of 17 kinds of amino acids. The protein has been shown to have antifertility action in male rats.
[中图分类号]
[基金项目]