20202203
20201125054311
zcyzzbjb@qq.comzcyzzbjb@qq.com
zcy
zcy
0253-2670
2020
51
22
Penicillium oxalicum, an endophytic fungus isolated from Pseudostellaria heterophylla]]>
2020
20202203
Pseudostellaria heterophylla (Miq) Pax;Penicillium oxalicum;endophytic fungus;secondary metabolites;oxafuranone A;benzofuran;isocoumarin;sesquiterpenoids;meroterpenoids]]>
目的 研究太子参Pseudostellaria heterophylla内生真菌草酸青霉Penicillium oxalicum的次级代谢产物。方法 采用正、反相硅胶柱色谱,葡聚糖凝胶Sephadex LH-20柱色谱以及制备薄层色谱(pTLC)等色谱技术进行系统分离纯化,采用质谱(MS)、核磁共振(NMR)等波谱学手段对单体化合物进行结构鉴定。结果 从草酸青霉发酵产物的醋酸乙酯萃取液中分离鉴定了12个化合物,包括1个香豆酮类化合物5,7-二羟基-2-甲基苯并呋喃-3-羧酸(1)、5个异香豆素类化合物(3R,4R)-(-)-4-hydroxymellein(2)、O-methylmellein(3)、acremonone G(4)、decarboxycitrinone(5)和decarboxyhydroxycitrinone(6),4个没药烷型倍半萜类化合物(7S,11S)-(+)-12-acetoxysydonic acid(7)、(S)-(+)-11-dehydrosydonic acid(8)、sydonic acid(9)和1-hydroxy-boivinianin A(10),2个混源萜类化合物decaturin D(11)和decaturin C(12)。结论 化合物1为新化合物,命名为草酸苯并呋喃酸A,化合物2~6、10为首次从草酸青霉中分离得到。]]>
Objective To study the secondary metabolites of Penicillium oxalicum, an endophytic fungus isolated from the medicinal plant Pseudostellaria heterophylla. Methods The secondary metabolites were isolated by silica gel, Sephadex LH-20 column chromatographies, and prep-TLC methods. Their structures were elucidated by using various spectroscopic techniques including HRESIMS and NMR spectra. Results A benzofuran compound 5,7-dihydroxy-2-methylbenzofuran-3-carboxylic acid (1), five isocoumarins including (3R,4R)-(-)-4-hydroxymellein (2), O-methylmellein (3), acremonone G (4), decarboxycitrinone (5) and decarboxyhydroxycitrinone (6), four bisabolane-type sesquiterpenoids including (7S,11S)-(+)-12-acetoxysydonic acid (7), (S)-(+)-11-dehydrosydonic acid (8), sydonic acid (9) and 1-hydroxy-boivinianin A (10), as well as two meroterpenoid-type alkaloids decaturin D (11) and decaturin C (12) were isolated. Conclusion Compound 1 is a new benzofuran compound and named as oxafuranone A, while compounds 2—6 and 10 are characterized from P. oxalicum for the first time.]]>
10.7501/j.issn.0253-2670.2020.22.003
20201125054311
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et al. Isocoumarins and benzofurans from the mangrove endophytic fungus Talaromyces amestolkiae possess α-glucosidase inhibitory and antibacterial activities[J]. RSC Adv, 2016, 6:26412.]]>
et al. Bioactive natural products from the endophytic fungus Ascochyta sp. from Meliotus dentatus-configurational assignment by solid-state CD and TDDFT calculations[J]. Eur J Org Chem, 2007, 2007(7):1123-1129.]]>
et al. Differential analysis of mycoalexins in confrontation zones of grapevine fungal pathogens by ultrahigh pressure liquid chromatography/time-of-flight mass spectrometry and capillary nuclear magnetic resonance[J]. J Agric Food Chem, 2009, 57(4):1127-1134.]]>
et al. Phthalide and isocoumarin derivatives produced by an Acremonium sp. isolated from a mangrove Rhizophora apiculata[J]. J Nat Prod, 2012, 75:853-858.]]>
Cercophora areolata[J]. J Nat Prod, 1996, 59(8):765-769.]]>
et al. Chemical constituents of a marine fungus, Arthrinium sacchari[J]. J Nat Prod, 2011, 74(7):1645-1649.]]>
et al. Cytotoxic polyphenols from the marine-derived fungus Penicillium expansum[J]. J Nat Prod, 2010, 73:910-914.]]>
Aspergillus sydowi[J]. Agric Biol Chem, 1978, 42(1):37-40.]]>
et al. Antimicrobial phenolic bisabolanes and related derivatives from Penicillium aculeatum SD-321, a deep sea sediment-derived fungus[J]. J Nat Prod, 2015, 78(4):844-849.]]>
et al. Antiinsectan decaturin and oxalicine analogues from Penicillium thiersii[J]. J Nat Prod, 2005, 68(3):319-322.]]>
Microbiol Mol Biol Rev, 2003, 67:491-502.]]>