目的 研究山胡椒Lindera glauca根的化学成分及其体外抗炎活性。方法 运用硅胶柱色谱、半制备高效液相色谱等分离技术进行分离纯化，通过高分辨电喷雾电离质谱、核磁共振、计算电子圆二色谱等波谱技术对化合物进行结构鉴定。以脂多糖（lipopolysaccharide，LPS）诱导的小鼠巨噬细胞RAW 264.7为模型，对化合物进行抗炎活性评价。结果 从山胡椒根95%乙醇提取物的石油醚部位分离得到11个化合物，分别鉴定为山胡椒萜素C（1）、glaucatotone H（2）、glaucatotone I（3）、glaucatotone G（4）、glaucatotone C（5）、glaucatotone K（6）、glaucatotone F（7）、glaucatotone L（8）、glaucatotone E（9）、glaucatotone D（10）、glaucatotone J（11）。化合物1～11在浓度为40 μmol/L时对RAW264.7细胞的NO生成抑制率在36.25%～87.62%，其中化合物6具有最强的抑制活性，抑制率达（87.62±4.21）%。结论 化合物1为新的二倍半萜，命名为山胡椒萜素C（1），其他化合物为愈创木烷型倍半萜类化合物。体外抗炎活性实验表明，愈创木烷型倍半萜对NO的释放具有不同程度的抑制活性。

Objective To investigate the chemical constituents from the roots of Lindera glauca and their in vitro anti-inflammatory activities. Methods The compounds were isolated and purified by many technologies, including silica gel column chromatography (CC) and semi-preparative high-performance liquid chromatography (HPLC). Their structures were elucidated by high resolution electrospray ionization mass spectrometry (HRESIMS), nuclear magnetic resonance (NMR), and calculated electronic circular dichroism (ECD), etc. The in vitro anti-inflammatory activity of the compounds were evaluated using lipopolysaccharide (LPS)-induced inflammatory model of RAW264.7 mouse macrophage cells. Results A total of 11 compounds were isolated from 95% ethanol extract of the roots of L. glauca and their chemcial structures were identified as linderasesterterpenoid C (1), glaucatotone H (2), glaucatotone I (3), glaucatotone G (4), glaucatotone C (5), glaucatotone K (6), glaucatotone F (7), glaucatotone L (8), glaucatotone E (9), glaucatotone D (10), glaucatotone J (11). For the NO production on RAW 264.7 cells, compounds 1—11 exhibited inhibitory rates from 36.25% to 87.62% at the concentration of 40 μmol/L, and compound 6 showed the strongest inhibitory activity with inhibitory ratio of (87.62 ±4.21)%. Conclusion Compound 1 is a new sesterterpenoid and named as linderasesterterpenoid C. Compounds 2—11 are guaiane-type sesquiterpenoids. The result of the in vitro anti-inflammatory activity showed that guaiane-type sesquiterpenoids had obvious inhibitory activity on the NO release.

R284.1

国家自然科学基金资助项目（32470428）