中药有机酸类成分种类繁多、化学结构多样且分布广泛，具有多种生理活性和药用价值。中药有机酸类成分的提取及分离纯化是后续药理药效、分子机制、新药研发等的研究基础与难点，目前相关研究的文献体量较大，涉及的提取方法包括热回流提取、超声辅助提取、微波辅助提取等及这些技术的联合使用；分离纯化主要依靠大孔树脂、硅胶、葡聚糖凝胶等传统柱色谱及色谱联用技术，及高效的色谱新材料、分子印迹技术、膜分离技术、高速逆流色谱技术等新型分离技术。目前研究主要集中于已有技术的拓展性使用、联合使用及工艺参数优化，未来应着力于开发及应用高效、绿色的提取及分离纯化新技术、新材料、新设备，聚焦解决中药有机酸类成分新药转化存在的瓶颈问题，为中药新药研发提供技术支撑。

Traditional Chinese medicine (TCM) had a wide variety of organic acid components with diverse chemical structures and widespread distribution, possessing various pharmacological activities and medicinal values. The extraction, separation and purification of organic acid components from TCM was the basis and difficulty of subsequent pharmacological activities, molecular mechanism, new drug development, etc. At present, there was a large volume of literature on related research, involving extraction methods such as heat reflux extraction, ultrasound-assisted extraction, microwave-assisted extraction, as well as the union of these techniques. The separation and purification of organic acid components from TCM mainly relied on traditional column chromatography such as macroporous adsorption resin, silica gel, dextran gel, and the union of chromatography techniques, as well as new separation technologies such as efficient new materials for chromatography, molecular imprinting technology, membrane separation technology, high speed counter-current chromatography. Current research mainly focused on the expansion use, joint use, and process parameter optimization of existing technologies. In the future, efforts should be made to develop and apply efficient and green new technologies, new materials, and new equipments of extraction, separation and purification, focusing on solving the bottleneck problems in new drug transformation of organic acid components from TCM, and providing technical support for the research and development of new drugs of TCM.

R284

国家自然科学基金资助项目（82460867）;甘肃省药品监督管理局药品监管科学研究项目（2023GSMPA046）;甘肃省中医药高水平重点课题（GZKZ-2024-16）;甘肃省人民医院中西医结合优势病种科技专项（23GSSYE-7）