目的 研究黄瑞香Daphne giraldii干燥根皮、茎皮的化学成分。方法 采用硅胶柱色谱、大孔吸附树脂、高效液相色谱等技术对黄瑞香干燥根皮、茎皮进行系统分离，综合应用理化常数、波谱光谱数据，鉴定了化合物的结构。采用改进的Ellman’s法测试了所有化合物的乙酰胆碱酯酶抑制活性。结果 从黄瑞香干燥根皮、茎皮的70%的乙醇提取物中分离获得8个化合物，包括6个香豆素及2个二芳基丙烷类化合物，分别鉴定为 (6R,7S)-6,7,10-trihydroxy-8,8-dimethyl-7,8-dihydro-2H,6H-pyrano[3,2-g]chromen-2-one（1a）、(6S,7R)-6,7,10-trihydroxy-8,8-dimethyl-7,8-dihydro-2H,6H-pyrano[3,2-g]chromen-2-one（1b）、10-hydroxy-8,8-dimethyl-2H,8H-pyrano[3,2-g]chromen-2-one（2）、花椒毒酚（3）、紫花前胡苷元（4）、8-methoxymarmesin（5）、1-(2'-hydroxy-4'-methoxyphenyl)-3-(4''-hydroxyphenyl)propane（6）、3-去氧苏木查耳酮（7）。结论 化合物1a/1b为新化合物，分别命名为黄瑞香素A和黄瑞香素B。化合物1b具有显著的乙酰胆碱酯酶抑制活性，与阳性药多奈哌齐活性相当。

Objective Chemical constituents of dried root bark and stem bark of Daphne giraldii. Methods Systematic isolation was performed using silica gel column chromatography, macroporous adsorption resin, and high-performance liquid chromatography (HPLC). The structures of the compounds were elucidated by physicochemical constants and spectroscopic data (UV, IR, MS, NMR). Acetylcholinesterase inhibitory activity of all compounds was evaluated using a modified Ellman's assay. Results Eight compounds were isolated from the 70% ethanol extract of D. giraldii root and stem bark, including six coumarins and two diarylpropanes. These compounds were identified as (6R,7S)-6,7,10-trihydroxy-8,8-dimethyl-7,8-dihydro-2H,6H-pyrano[3,2-g]chromen-2-one (1a), (6S,7R)-6,7,10-trihydroxy-8,8-dimethyl-7,8-dihydro-2H,6H-pyrano[3,2-g]chromen-2-one (1b), 10-hydroxy-8,8-dimethyl-2H,8H-pyrano[3,2-g]chromen-2-one (2), xanthotoxol (3), decursinol (4), 8-methoxymarmesin (5), 1-(2'-hydroxy-4'-methoxyphenyl)-3-(4''-hydroxyphenyl)propane (6), and 3-deoxysappanchalcone (7). Conclusion Compounds 1a and 1b are novel structures named daphnetin A and daphnetin B. Compound 1b exhibited significant acetylcholinesterase inhibitory activity, comparable to that of the positive control donepezil.

R284.1

国家自然科学基金项目（81872766）