目的 采用方证代谢组学方法分析三妙丸干预湿热下注型痛风病的药效物质基础。方法 采用非靶向代谢组学技术筛选湿热下注型痛风病的生物标记物，利用超高效液相色谱与质谱联用技术分析显效状态下三妙丸的血中移行成分，并将其疾病生物标记物关联，确定三妙丸干预湿热下注型痛风病的药效物质基础。结果 三妙丸能够显著回调湿热下注型痛风病小鼠的代谢轨迹，改善模型小鼠湿热证表现及痛风性关节炎情况，降低踝关节肿胀度，抑制NOD样受体热蛋白结构域3、白细胞介素-6（interleukin-6，IL-6）、IL-1β炎症因子表达，方证代谢组学分析显示，白术内酯III、白术内酯II、牛膝皂苷C、京尼平苷、小檗碱、皱唐松草酮碱、亚美罂粟碱、榄香烯、苦楝子酮、油酸为三妙丸干预的湿热下注型痛风病的潜在药效物质基础。结论 利用方证代谢组学方法揭示了三妙丸干预的湿热下注型痛风病的药效物质基础，为三妙丸的精准用药和湿热下注型痛风病治疗药物的开发提供理论依据。

Objective To analyze the pharmacological substance basis of Sanmiao Pills (三妙丸) in intervening damp-heat type gout by using the chinmedomics. Methods Biomarkers of damp-heat type gout were screened by non-targeted metabolomics technology, and ultra-high performance liquid chromatography and mass spectrometry technology were used to analyze the constituents absorbed into blood of Sanmiao Pills in the manifested state, and the disease biomarkerswere correlated to determine the pharmacological substance basis of Sanmiao Pills in intervening damp-heat type gout.Results Sanmiao Pills was able to significantly regulate the metabolic profile of mice with damp-heat type gout, improve the manifestation of damp-heat syndrome and gouty arthritis in the model mice, reduce ankle swelling, and inhibit the expression of the NOD-like receptor thermoprotein structural domain 3, interleukin-6 (IL-6), and IL-1β inflammatory factors, and chinmedomics analysis showed that atractylenolide III, atractylenolide II, achyranthoside C, geniposide, berberine, rugosinone, evoeuropine, β-elemene, melianone, and oleic acid were the potential pharmacological substance bases of damp-heat type gout intervened with the Sanmiao Pills.Conclusion The chinmedomics was used to reveal the pharmacological substance basis of damp-heat type gout treated with Sanmiao Pills, which provides a theoretical basis for the precise administration of Sanmiao Pills and the development of therapeutic drugs for the treatment of damp-heat type gout.

R285

国家自然科学基金区域创新发展联合基金项目（U23A20501）;省部共建中医湿证国家重点实验室项目（SZ2021ZZ49）;省部共建中医湿证国家重点实验室项目（SZ2022KF16）