目的 阐明云南白药配伍环境中草乌Aconiti Kusnezoffii Radix活血化瘀作用的药效物质基础，科学评价草乌在云南白药配伍环境中的作用。方法 应用方证代谢组学研究策略，在应用临床生物化学及组织病理学评价云南白药治疗血瘀证的有效性的基础上，利用代谢组学技术发现云南白药治疗血瘀证调节的血液代谢标记物及代谢通路，进一步应用代谢标记物与血中成分关联分析挖掘与血液代谢标记物高度相关的入血成分。结果 云南白药能改善血瘀证模型大鼠血液流变学参数及组织病理学改变，并将血瘀证大鼠的23个血液代谢标记物回调至正常水平，其中13个具有统计学意义（P＜0.05、0.01）。在云南白药治疗血瘀证有效状态下表征的7个草乌生物碱类血中移行成分中，4个入血成分与血液代谢标记物高度相关，最终确定森布星B、异塔拉定、塔拉乌头胺及展花乌头宁为云南白药配伍环境中草乌活血化瘀作用的潜在药效物质基础。结论 利用方证代谢组学技术揭示了云南白药配伍环境中草乌活血化瘀作用的药效物质基础研究，为精准评价云南白药疗效，实现云南白药临床精准遣药、产品升级并进一步提升认可度及市场占有率服务。

Objective To elucidate the pharmacodynamic material basis of activating blood circulation and removing blood stasis effect of Caowu (Aconiti Kusnezoffii Radix) in Yunnan Baiyao (云南白药, YNBY) compatibility environment, and interpret the effects of Aconiti Kusnezoffii Radix in YNBY compatibility environment. Methods Chinmedomics strategy was applied to the study. On the basis of clinical biochemistry and histopathology evaluation of the effectiveness of YNBY in the treatment of blood stasis syndrome, the metabolic markers and metabolic pathways regulated by YNBY in treatment of blood stasis syndrome were found by metabolomics. Analysis of correlation between metabolic markers and serum constituents was used to excavate blood entry components that were highly correlated with blood metabolic markers. Results YNBY could improve hemorheological parameters and histopathological changes of blood stasis syndrome model rats, and restore 23 blood metabolic markers to normal level in rats with blood stasis syndrome, 13 of them were statistically significant (P < 0.05, 0.01). A total of seven alkaloids components of Aconiti Kusnezoffii Radix absorbed into blood in the effective state of YNBY treatment of blood stasis syndrome were characterized, four of them were highly correlated with blood metabolic markers. It was finally determined that sembuxin B, isotalatizidine, talatisamine and chasmanine were the potential pharmacodynamic material bases of Aconiti Kusnezoffii Radix in the compatibility environment of YNBY in promoting blood circulation and removing blood stasis. Conclusion This study determined the potential pharmacodynamic material bases of Aconiti Kusnezoffii Radix in the compatibility environment of YNBY in promoting blood circulation and removing blood stasis, which serves to effective evaluation of YNBY, clinical precision dispensing and product upgrading of YNBY, and further enhance the recognition and market share.

R285.5

国家中医药管理局公益性行业科研专项（2015468004）;国家自然科学基金面上项目（82274199）;黑龙江省自然科学基金青年基金项目（QC2018117）;中央支持地方高校改革发展资金人才培养计划（2021ZYYQLG001）;黑龙江科技英才春雁支持计划（CYQN24013）