证候与方剂是中药有效性相关的两大科学问题。方剂是临床使用药物形式，证候是对疾病的总体描述。方剂多药物组成的复杂性以及中医证候诊断标准的模糊性，制约着方剂效应的系统性评价、药效物质基础的确认、方剂疗效机制的科学阐释。方证代谢组学（chinmedomics）融合中医药理论与现代科学技术，通过整合中药血清药物化学与多组学关键技术，实现揭示证候生物学本质，构建方剂疗效精准评价体系，从而发现与临床疗效相关的方剂体内药效物质基础，阐明方剂作用机制，将有效性说清楚、讲明白并用的上。综述方证代谢组学理论与方法的形成，以及近年来该理论方法在中医药研究领域的应用和发展，以促进方证代谢组学在中药现代研究中更广泛的应用。

Syndrome and formula are two major scientific issues related to the effectiveness of traditional Chinese medicine (TCM). TCM formulas are the drug form of clinical use, and the syndrome is an overall description of the disease. Due to the complex chemical components in TCM formulas and the fuzziness of diagnostic criteria for TCM syndromes, it restricts the systematic evaluation of the effects of TCM formulas, the confirmation of the active ingredients, and the scientific interpretation of the mechanism of formula efficacy. Chinmedomics integrates TCM theory with modern scientific technology, it combines serum pharmacochemistry of TCM and multi omics techniques to reveal the biological essence of syndromes, establish an accurate formula efficacy evaluation system, discover the in vivo active ingredients, and reveal the mechanism of therapeutic effect aiming to clearly explain the effectiveness of TCM and apply it effectively. This article summarizes the formation of chinmedomics theory and methods, as well as the application and development of this theory and method in the field of TCM research in recent years, in order to promote the wider application of chinmedomics in modern Chinese medicine research.

R285

国家自然科学基金区域创新发展联合基金项目（U23A20501）;国家自然科学基金专项课题（32141005）;黑龙江省重点研发计划项目（2022ZX02C04）