目的 研究坚叶樟Camphora chartophylla醋酸乙酯部位的化学成分。方法 采用硅胶柱色谱、Sephadex LH-20柱色谱和半制备HPLC等手段进行分离纯化，通过核磁共振波谱、质谱、红外光谱鉴定化合物的结构。同时，通过MTT和DPPH法评价化合物的细胞毒性和体外抗氧化作用。结果 从坚叶樟乙醇提取物中分离得到10个化合物，分别鉴定为(1S,2R)-1-(4-(((1R,2R)-1,3-dihydroxy-1-(4-hydroxy-3-methoxyphenyl)propan-2-yl)oxy)-3-methoxyphenyl)-2-(4-hydroxy-3-methoxy- phenyl)propane-1,3-diol（1）、leptolepisol D（2）、(＋)-(8R,7¢S,8¢R)-9¢-O-(E)-cinnamoyl-5,5¢-dimethoxylariciresinol（3）、(7R,8S)-3,5¢-dimethoxy-4¢,7-epoxy-8,3¢-neolignane-5,9,9¢-triol（4）、2,6,2¢,6¢-tetramethoxy-4,4¢-bis(2,3-epoxy-1-hydroxypropyl) biphenyl（5）、南烛木糖苷（6）、裸柄吊钟花脂苷（7）、2S-3-(4-hydroxy-3-methoxyphenyl)propane-1,2-diol（8）、吐叶醇（9）和桉油烯醇（10）。化合物1～10对RAW264.7细胞活力无明显的抑制作用，化合物1、2、5和7显示一定的抗氧化活性。结论 化合物1为新化合物，命名为坚木樟素A；化合物1～10为首次从坚叶樟中分离得到，化合物1、2、4、6～8为首次从樟属中分离得到。化合物1、2、5和7具有抗氧化作用。

Objective To study the chemical composition of acetic ether part of Camphora chartophylla. Methods The compounds were isolated and purified by silica gel column chromatography (CC), Sephadex LH-20 CC, and semi-preparative high-performance liquid chromatography (HPLC). Their structures were elucidated by NMR, MS, and IR spectroscopy. All isolated compounds were subjected to the evaluation of cytotoxity and antioxidant activity in vitro using MTT and DPPH methods. Results Ten compounds were isolated and identified as (1S,2R)-1-(4-(((1R,2R)-1,3-dihydroxy-1-(4-hydroxy-3-methoxyphenyl)propan-2-yl)oxy)-3-methoxyphenyl)-2-(4-hydroxy-3-methoxy- phenyl)propane-1,3-diol (1), leptolepisol D (2), (＋)-(8R,7¢S,8¢R)-9¢-O-(E)-cinnamoyl-5,5¢-dimethoxylariciresinol (3), (7R,8S)-3,5¢-dimethoxy-4¢,7-epoxy-8,3¢-neolignane-5,9,9¢-triol (4), 2,6,2¢,6¢-tetramethoxy-4,4¢-bis(2,3-epoxy-1-hydroxypropyl)biphenyl (5), lyoniside (6), nudiposide (7), 2S-3-(4-hydroxy-3-methoxyphenyl)propane-1,2-diol (8), vomifoliol (9) and spathulenol (10). All compounds exhibited no significant inhibitory effect on cell viability and compounds 1, 2, 5, and 7 exhibited certain antioxidant activity. Conclusion Compound 1 is a new compound named chartane A. All compounds are isolated from C. chartophyll for the first time and compounds 1, 2, 4, and 6—8 are isolated from the genus Cinnamomum for the first time. Compounds 1, 2, 5, and 7 exhibited antioxidant activities.

R284.1

国家自然科学基金项目（82204762）；山东省中医药科技发展计划项目（2020M037）山东省中医药高层次人才培育项目；国家中医药管理局科技司-山东省卫生健康委员会共建中医药科技项目（GZY-KJS-SD-2023-051）