目的 阐明番泻叶Sennae Folium的化学成分，并对主要化合物进行定量分析，为番泻叶质量评价提供依据。方法 采用超高效液相色谱-四极杆-静电场轨道阱高分辨质谱（UHPLC-Q-exactive-orbitrap-MS）负离子模式对番泻叶中主要化学成分进行快速鉴定；采用UPLC-PDA法对6个主要化合物进行定量分析，并应用于多批次样品测定。结果 番泻叶鉴定了29个化合物，其中蒽醌类化合物12个、黄酮类化合物11个、二苯甲酮类化合物2个及萘类化合物4个；测定了20批番泻叶样品中6个主要成分（新西兰牡荆苷、芦荟大黄素-8-O-葡萄糖苷、番泻苷A、番泻苷B、番泻苷C、丁内未利葡萄糖苷）的含量，不同批次中各化合物含量存在差异。结论 对番泻叶中化学成分进行快速鉴定和主要成分定量分析，为番泻叶的质量评价提供依据。

Objective To clarify the chemical components and quantify the main components, so as to provide the basis for the quality evaluation of Fanxieye (Sennae Folium). Methods UHPLC-Q-Exactive-Orbitrap-MS was employed to quickly identify the main chemical components of Sennae Folium in negative ion mode. Furthermore, the quantitative analysis of six main chemical components was accomplished by ultra performance liquid chromatography with photo-diode array (UPLC-PDA) detection in Sennae Folium. Results A total of 29 components were identified, including 12 anthraquinones, 11 flavonoids, two benzophenones and four naphthalenes. The content of six main components (vicenin 2, aloe emodin-8-O-glucoside, sennoside A, sennoside B, sennoside C, and tinnevellin glucoside) were determined in 20 batches Sennae Folium, which varied obviously in different batches. Conclusion The rapid identification of chemical components and the quantitative analysis of six components were successfully accomplished in Sennae Folium, which provided the basis for the quality evaluation of Sennae Folium.

R286.2

国家中医药管理局中药标准化项目（ZYBZH-C-JX-38）