目的 基于“辨色论质”理论对箭叶淫羊藿Epimedium sagittatum叶片色泽与其淫羊藿苷类有效成分进行关联分析，为箭叶淫羊藿“辨色论质”质量评价体系的建立提供理论参考。方法 使用色差法测定103批箭叶淫羊藿叶片表观色泽指标（明度值L^*、红绿度值a^*、黄蓝度值b^*、总色差E^*）；HPLC法测定叶片中朝藿定A、朝藿定B、朝藿定C、淫羊藿苷、宝藿苷I含量；紫外分光光度法测定叶片中总黄酮含量；运用SPSS软件进行差异分析、K-聚类分析、相关性分析、回归分析。结果 103批箭叶淫羊藿叶片L^*为33.38～48.22、a^*为−7.56～−3.50、b^*为9.17～28.68、E^*为36.82～56.38，可见其表观色泽有显著差异；其有效成分定量分析显示，淫羊藿叶片中朝藿定A质量分数为0.28～3.55 mg/g、朝藿定B为0.20～3.60 mg/g、朝藿定C为11.10～124.51 mg/g、淫羊藿苷为0.28～30.55 mg/g、宝藿苷I为0.28～7.32 mg/g、总黄酮为26.72～169.12 mg/g；经K-聚类分析将箭叶淫羊藿叶片分为Ⅰ～Ⅴ类，其中第Ⅲ类叶片整体色差均一，呈现亮黄绿色稀黄色斑点，有效成分含量相较于其他4类处于最高水平；经相关性分析表明总黄酮和其他5种有效成分含量与L^*、b^*、E^*呈显著正相关（P＜0.05），与a^*呈显著负相关（P＜0.05）；经回归分析得淫羊藿苷与色泽指标的回归方程为Y＝0.009 269＋0.000 236L^*＋0.000 881a^*＋0.000 214b^*。结论 箭叶淫羊藿叶片色泽与有效成分含量高度关联，可为箭叶淫羊藿叶片有效成分含量快速预测及“辨色论质”评价体系的构建提供参考依据。

Objective Based on the theory of “quality discrimination by color characteristic”, the correlation between the leaves color and the effective components of icariin was analyzed in order to provide theoretical reference for the establishment of the quality evaluation system of “quality discrimination by color characteristic” of Epimedium sagittatum. Methods The apparent color indexes (brightness value L^*, redness value a^*, yellowness chromaticity value b^*, and total color difference E^*) of 103 batches of E. sagittatum leaves were determined by the color difference method. The contents of epimedin A, epimedin B, epimedin C, icariin and baohuoside I were determined by HPLC and the total flavonoid contents were determined by UV spectrophotometry in the leaves. Analysis of variance, K-cluster analysis, correlation analysis, and regression analysis were performed by SPSS software. Results The apparent color indexes of L^*, a^*, b^* and E^*of 103 batches of E. sagittatum leaves were in the range of 33.38—48.22, −7.56—−3.50, 9.17—28.68, and 36.82—56.38, respectively, which showed that there was a significant difference in their apparent coloration; Quantitative analysis of the effective components showed that the content of epimedin A, epimedin B, epimedin C, icariin, baohuoside I, total flavonoids in the leaves of E. sagittatum was in the range of 0.28—3.55 mg/g, 0.20—3.60 mg/g, 11.10—124.51 mg/g, ranging from 0.28—30.55 mg/g, 0.28—7.32 mg/g, and ranged from 26.72—169.12 mg/g, respectively. After K-cluster analysis, the leaves of E. sagittatum were divided into Ⅰ—Ⅴ types, in which the leaves of the Ⅲ type were of uniform color, showing bright yellow-green and diluted yellow spots, and the content of active ingredients was at the highest level compared with the other four types. The correlation analysis showed that the total flavonoids and the other five active ingredients were positively correlated with L^*, b^*, and E^* (P < 0.05), and were negatively correlated with a^* (P < 0.05); According to the regression analysis, the regression equation between icariin and color index was Y = 0.009 269 + 0.00 0236L^* + 0.000 881a^* + 0.000 214b^*.Conclusion The results of this study show that the color of the leaves of E. sagittatum is highly correlated with the content of active ingredients, which can provide a reference basis for the rapid prediction of the content of active ingredients in the leaves of E. sagittatum and the construction of the evaluation system of “quality discrimination by color characteristic”.

R286.2

中国博士后科学基金特别资助项目（2014T170252）;国家自然科学基金资助项目（82104329，32401226）;河南省重点研发专项（231111312700，241111310200）;河南省国际科技合作项目（242102521056）;河南省科技攻关项目（242102310549）