目的 对直立百部Stemona sessilifolia块根的化学成分进行分离鉴定。方法 采用硅胶、Sephadex LH-20、ODS等柱色谱及制备型高效液相等方法进行分离纯化；根据化合物的波谱数据（MS、¹H-与¹³C-NMR）鉴定其结构；利用CCK-8法及Griess法测定化合物的细胞毒活性及抗炎活性（NO含量）。结果 从直立百部块根的乙醇提取物中分离得到8个酚类化合物，分别鉴定为4-氯-3-甲氧基-5-甲基苯酚-1-O-β-D-呋喃芹糖基-(1→6)-β-D-吡喃葡萄糖苷（1）、4-氯-3-甲氧基-5-甲基苯基- 1-O-α-L-吡喃鼠李糖基-(1→6)-β-D-吡喃葡萄糖苷（2）、3,4,5-三甲氧基苯基-1-O-β-D-吡喃葡萄糖苷（3）、刺五加苷B（4）、肿柄雪莲苷（5）、(＋)-异落叶松树脂醇-3α-O-β-D-吡喃葡萄糖苷（6）、去氢-δ-生育酚（7）与去氢-γ-生育酚（8）。结论 化合物1为1个新化合物，化合物2～6为首次从百部属植物中分离得到，7和8为首次从直立百部中分离得到；化合物3、4、7和8对RAW 264.7细胞没有明显的细胞毒性，存活率均大于85%，其具有弱的抗炎活性，在20 μmol/L下对脂多糖诱导的RAW 264.7细胞炎症因子NO释放的抑制率在20%～50%。

Objective To study chemical constituents in the Stemona sessilifolia roots with their anti-inflammatory activities. Methods Compounds were isolated and purified by various chromatography, including silica gel, ODS, Sephadex LH-20 and preparative HPLC. Their structures were elucidated by spectroscopic data analysis. Cytotoxicity and anti-inflammatory activities were evaluated by CCK-8 and Griess experiments. Results Eight compounds were obtained from the tuber roots of S. sessilifolias and identified as 4-chloro-3-methoxy-5-methylphenol-1-O-β-D-apiofuranosyl-(1→6)-β-D-glucopyranoside (1), 4-chloro-3-methoxy-5-methylphenyl-1-O-α-L-rhanopyranosyl-(1→6)-β-D-glucopyranoside (2), 3,4,5-trimethoxyphenyl-1-O-β-D-glucopyranoside (3), eleutheroside B (4), conicaoside (5), (+)-isolariciresinol-3α-O-β-D-glucopyranoside (6), dehydro-δ-tocopherol (7) and dehydro-γ-tocopherol (8). Conclusion Compound 1 is a new natural product, compounds 2―6 were isolated from Stemona genus for the first time, and compounds 7 and 8 were obtained firstly from S. sessilifolias. Compounds 3, 4, 7 and 8 showed no cytotoxicity on RAW 264.7 cells and weak anti-inflammatory activities all with 20%―50% NO inhibition at 20 μmol/L.

R284.1

国家中医药管理局科技司−山东省卫生健康委员会共建中医药科技项目(GZY-KJS-SD-2023-087)