目的 探讨苍艾挥发油（Cangai volatile oil，CAVO）干预哮喘小鼠经典瞬时受体电位通道1（transient receptor potential canonical channel 1，TRPC1）信号通路介导内质网应激对气道重塑的影响。方法 将36只Balb/c小鼠随机分对照组、模型组、地塞米松（1.50 mg/kg）组及CAVO高、中、低剂量（3.86、1.93、0.97 mg/kg）组。除对照组外，其余各组小鼠采用卵清蛋白（ovalbumin，OVA）致敏、激活建立小鼠哮喘模型，各给药组分别ig相应药物（20 mL/kg），对照组、模型组ig等体积生理盐水，1次/d，连续给药6周后，处死小鼠剖取肺组织，Western blotting检测蛋白激活转录因子6（activating transcription factor 6，ATF6）、蛋白激酶RNA样内质网激酶（protein kinase RNA-like endoplasmic reticulum kinase，PERK）、C/EBP同源蛋白（C/EBP homologous protein，CHOP）、I型胶原蛋白（Collagen type I，Collagen I）、Collagen III的表达水平。选取SD大鼠20只制备含药血清，按照体表面积计算给药剂量，2次/d，连续给药7 d，于末次给药1 h后处死大鼠，取血、处理备用；收集哮喘小鼠肺泡灌洗液并纯化分离肺泡巨噬细胞，随机分为对照组、5% CAVO含药血清组、5% CAVO含药血清＋空载组、5% CAVO含药血清＋TRPC1过表达组，与人支气管上皮样细胞16HBE共培养，收集细胞，采用Western blotting检测各组细胞ATF6、PERK、CHOP、Collagen I、Collagen III、TRPC1的蛋白表达，采用定量逆转录聚合酶链反应（quantitative reverse transcription polymerase chain reaction，qRT-PCR）比较各组Collagen I、Collagen III、TRPC1的表达。结果 体内实验发现，与对照组比较，模型组的ATF6、PERK、CHOP、Collagen I及Collagen III的表达显著上升（P＜0.01）；与模型组比较，CAVO高、中、低剂量组ATF6、PERK、CHOP、Collagen Ⅰ、Collagen Ⅲ蛋白表达水平显著下降（P＜0.05）。体外实验发现，与对照组比较，5% CAVO含药血清组、5% CAVO含药血清＋空载组中的ATF6、PERK、CHOP、Collagen I、Collagen III、TRPC1的表达水平显著下降（P＜0.01）；与5% CAVO含药血清＋空载组比较，5% CAVO含药血清+TRPC1过表达组中的ATF6、PERK、CHOP、Collagen I、Collagen III、TRPC1的表达水平显著上升（P＜0.05）。结论 CAVO对哮喘小鼠气道重塑和气道炎症有改善作用，这可能与调节TRPC1信号通路，影响内质网应激反应，改善哮喘气道重塑有关。

Objective To investigate the effect of Cangai volatile oil (CAVO) on airway remodelling mediated by endoplasmic reticulum stress in asthmatic mice by intervening in the classical transient receptor potential canonical channel 1 (TRPC1) signalling pathway.Methods A total of 36 Balb/c mice were randomly divided into control group, model group, dexamethasone (1.50 mg/kg) group, and CAVO high-, medium- and low-dose (3.86, 1.93, 0.97 mg/kg) groups. In addition to control group, the rest of the groups of mice were sensitised by ovomucoid protein (OVA) to establish an asthma model. Each group ig corresponding drug (20 mL/kg) respectively, and control group and model group ig an equal amount of saline, once a day, for six weeks. After six weeks of administration, lung tissues were dissected for Western blotting to observe protein activating transcription factor 6 (ATF6), protein kinase RNA-like endoplasmic reticulum kinase (PERK), C/EBP homologous protein (CHOP), Collagen type I (Collagen I), Collagen III expression levels. A total of 20 SD rats were selected to prepare drug-containing serum, and the dosage of drug administered to SD rats was calculated according to the body surface area, and the drug was administered by gavage for 7 d, twice a day, and the rats were executed 1 h after the last administration, and the blood was taken and processed for spare parts. The alveolar lavage fluid of asthmatic mice was collected and purified to isolate alveolar macrophages, which were co-cultured with human bronchial epithelial cells 16HBE, and randomly divided into control group, 5% CAVO-containing serum group, 5% CAVO-containing serum + null-loaded group, and 5% CAVO-containing serum + TRPC1-overexpression group. Cells were collected and protein expression of ATF6, PERK, CHOP, Collagen Ⅰ, Collagen Ⅲ and TRPC1 were detected by Western blotting, the expression of Collagen Ⅰ, Collagen Ⅲ and TRPC1 in each group were detected by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Results In vivo experiments revealed that the expression of ATF6, PERK, CHOP, Collagen I and Collagen III in the model group was significantly increased compared with the control group (P < 0.01), compared with the model group, the protein expression levels of ATF6, PERK, CHOP, Collagen I, and Collagen III in the CAVO high-, medium-, and low-dose group were significantly decreased (P < 0.05). In vitro experiments revealed that the expression levels of ATF6, PERK, CHOP, Collagen I, Collagen III, and TRPC1 were significantly decreased in the 5% CAVO-containing serum group and the 5% CAVO-containing serum + null group compared with the control group (P < 0.01), and in the 5% CAVO-containing serum + TRPC1-overexpression group, the expression levels of expression levels of ATF6, PERK, CHOP, Collagen I, Collagen III, and TRPC1 were significantly increased compared with 5% CAVO-containing serum + null-loaded group (P < 0.05).Conclusion CAVO treatment of asthma may be related to regulating the TRPC1 signaling pathway, affecting the endoplasmic reticulum stress response, and improving asthma airway remodeling.

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国家自然科学基金资助项目（82060884）;云南省基础研究计划重点项目（202401AS070809）;云南省兴滇英才支持计划-医疗卫生人才专项（XDYC-YLWS-2023-0092）;云南省万人计划青年拔尖人才专项（YNWR-QNBJ-2019-196）;国家中医药管理局高水平中医药重点学科建设项目（ZYYZDXK-2023190）;熊磊全国名老中医药专家传承工作室项目