目的 研究垫状卷柏Selaginella pulvinata全草的化学成分。方法 采用硅胶、Sephadex LH-20等多种柱色谱方法进行分离纯化，应用理化方法和波谱分析进行结构鉴定；采用MTT法测定化合物对人非小细胞肺癌A549细胞的抑制活性。结果 从垫状卷柏全草的95%乙醇提取物的醋酸乙酯部位分离得到1个新骨架类型的炔酚类化合物，鉴定为9-羟基-3,4,13-三(4-羟基苯基)-7H-二苯并[gh,pqr]四苯基-7-酮（1）；化合物1对A549细胞显示出较强的抑制活性，其半数抑制浓度（half maximal inhibitory concentration，IC₅₀）为（16.29±1.44）μg/mL。结论 化合物1为新化合物，命名为卷柏新酮，具有选择性细胞毒活性。

Objective To study the chemical constituents of the whole plants of Selaginella pulvinata. Methods The chemical constituents were isolated and purified by silica gel and Sephadex LH-20 column chromatography. The structures of the isolated compounds were identified based on physicochemical properties and spectroscopic data, and the cytotoxic activities against tested human cancer cell lines were evaluated by MTT method. Results A novel selaginellin derivative was isolated from the whole plants of S. pulvinata, which was identified as 9-hydroxy-3,4,13-tris(4-hydroxyphenyl)-7H-dibenzo[gh,pqr]tetraphen-7-one (1). Compound 1 showed potent inhibitory activity against human non-small cell lung cancer A549 cell with its half maximal inhibitory concentration of (16.29 ±1.44) μg/mL. Conclusion Compound 1 is a novel compound, named selaginbenzopyrene A, which shows selective cytotoxic activity.

R284.1

国家自然科学基金项目(82073983)