目的 以南洋参Polyscias fruticose和线叶南洋参P. filicifolia的悬浮培养细胞为研究对象，研究在细胞培养过程中，丙二酰基人参皂苷（malonyl ginsenoside，M-GS）对细胞生长及皂苷合成的影响，以促进南洋参悬浮培养细胞的皂苷合成和积累。方法 以南洋参和线叶南洋参的细胞系（编号6a和VDK）为试验材料，在悬浮培养的第7天添加M-GS，分析添加M-GS对细胞生长以及细胞中多糖苷E、人参皂苷R0、多糖苷A和齐墩果酸皂苷A含量的影响。结果 M-GS对南洋参和线叶南洋参的生长均具有抑制作用，使其不能长期生存。M-GS可使南洋参6a细胞系的细胞密度以及干质量峰值提前出现，使细胞活力提前开始下降，缩短了细胞的培养周期。而线叶南洋参VDK细胞系的细胞密度、鲜质量以及干质量的峰值延迟出现，延长了细胞的培养周期。M-GS对南洋参6a细胞系的抑制作用比线叶南洋参VDK更强。在培养末期南洋参6a细胞系的细胞密度、活力、鲜质量以及干质量分别降低了25.43%、22.23%、46.56%以及45.56%。M-GS对南洋参和线叶南洋参细胞中皂苷积累的影响不同。对于南洋参6a细胞系，M-GS促进细胞中多糖苷E、多糖苷A、齐墩果酸皂苷A的合成，且对多糖苷E的促进能力最强（提高了79.67%），其次为齐墩果酸皂苷A（提高了70.67%），最后为多糖苷A（提高了19.67%），同时抑制了人参皂苷R0的含量（降低了74.00%）。对于线叶南洋参VDK细胞系，M-GS只促进了细胞中多糖苷A的合成，使其含量增加了63.29%，却抑制了细胞中多糖苷E、多糖苷A、齐墩果酸皂苷A的合成，且对多糖苷A抑制能力最强（降低了63.29%），其次为多糖苷E（减低了27.59%），最后为齐墩果酸皂苷A（减低了8.33%）。结论 添加M-GS可以促进南洋参和线叶南洋参悬浮培养细胞的部分种类皂苷积累，但不宜于细胞长期培养；且M-GS对不同物种细胞系细胞中不同皂苷的影响不同，对南洋参6a细胞系的促进能力高于线叶南洋参VDK细胞系。因此在南洋参和线叶南洋参细胞悬浮培养中可根据培养细胞系和目的产物不同选择使用M-GS以提高目的皂苷产量。

Objective With suspension cultured cells of Polyscias fruticosa and P. filicifolia as research objects, we explored the effects of malonyl ginsenosides (M-GS) on the cell growth and saponin synthesis during the cell culture to promote the synthesis and accumulation of saponin in suspension cultured cells of P. fruticosa. Methods Cell lines (6a and VDK) of P. fruticosaand P. filicifolia were used as experimental materials. M-GS was added on 7th day of the suspension culture to analyze the effects of M-GS addition on the cell growth and contents of polyscioside E, ginsenoside R0, polyscioside A and ladyginoside A saponins in cells.Results M-GS could inhibit the growth of P. fruticosa and P. filicifolia, preventing their long-term survival. M-GS brought forward the peak of cell density and dry weight of 6a cell line of P. fruticosa, causing the cell viability to start declining earlier, and shortening the cell culture cycle. While the peak values of cell density, fresh weight and dry weight of VDK cell line were delayed, which prolonged the cell culture cycle. The inhibitory effect of M-GS on 6a cell line was stronger than that of VDK. The cell density, vitality, fresh weight and dry weight of 6a cell line decreased by 25.43%, 22.23%, 46.56% and 45.56%, respectively. The effects of M-GS on the accumulation of saponins in the cells of P. fruticosa and P. filicifolia were different. For the cell line 6a of P. fruticosa, M-GS promoted the synthesis of Polyscioside E, Polyscioside A and Ladyginoside A saponins, and the promotion was the strongest for Polyscioside E saponins (increased by 79.67%), followed by ladyginoside A saponins (increased by 70.67%) and polyscioside A saponins (increased by 19.67%). However, the content of ginsenoside R0 saponins was inhibited (reduced by 74.00%). For the VDK cell line of Panax lineolata, M-GS only promoted the synthesis of Polyscioside A saponins, increasing its content by 63.29%, but inhibited the synthesis of polyscioside A, Polyscioside A and Ladyginoside A saponins, and had the strongest inhibition on polyscioside A saponins (decreased by 63.29%), followed by Polyscioside A saponins (decreased by 27.59%) and Ladyginoside A saponins (decreased by 8.33%). Conclusion The addition of malonyl ginsenosides can promote the accumulation of some kinds of saponins in suspension cultured cells of P. fruticosa and P. filicifolia, but it is not suitable for the long-term cell culture. Moreover, the effects of malonyl-ginsenosides on different saponins in different cell lines were different, with a higher promotional ability on 6a cell line than on the VDK cell line. Therefore, malonyl ginsenosides can be selected to increase the yield of target saponins in cell suspension culture of P. fruticosa andP. filicifolia according to different cell lines and target products.

R286.2

国家重点研发计划（2023YFD2200103）