目的 分析裸花紫珠Callicarpa nudiflora种质资源遗传变异情况，发掘与有效成分含量相关联的分子标记，以期为裸花紫珠新品种选育提供参考。方法 对103份裸花紫珠种质资源有效成分含量进行变异性分析，并利用14对SSR标记对供试品进行遗传结构分析及连锁不平衡分析，在此基础上采用Talssel 2.1软件的一般线性模型（GLM）和混合线性模型（MLM）进行SSR标记与有效成分含量的关联分析。结果 裸花紫珠中7种有效成分含量变异丰富，可作为关联分析的理想材料。群体遗传结构分析结果表明，K＝3时，供试品分为3个亚群，3个亚群的遗传多样性均较为丰富，AMOVA分析显示变异主要存在于个体内（61%）。在91个SSR位点成对组合中，不论共线性组合还是非共线性组合均存在一定程度的连锁不平衡（LD）。D'统计概率（P＜0.01）支持的LD成对位点有21个，占全部位点组合的23.08%。D'值主要集中在0.3～0.5和0.5～0.7，占SSR标记全部对数的84.62%，表明所选种质间基因重组概率与突变概率较低。关联分析结果表明，在GLM模型中，发现有11对标记与7种有效成分含量呈显著相关（P＜0.05）或极显著相关（P＜0.01）。在MLM模型中，6对标记与4种有效成分含量呈显著相关（P＜0.05）或极显著相关（P＜0.01）。在GLM和MLM模型中，均检测到CnH305与连翘酯苷B和木犀草苷相关联（P＜0.05），CnH155与木犀草苷和毛蕊花糖苷相关联（P＜0.01），CnH156、CnH357和CnH205与异毛蕊花糖苷相关联。结论 供试裸花紫珠种质存在丰富的遗传变异，种质间存在一定程度的连锁不平衡，5对SSR标记能够很好的关联到药效成分含量，可以为裸花紫珠分子标记辅助育种提供理论依据。

Objective The genetic variation of Callicarpa nudiflora germplasm resources was analyzed, and the molecular markers associated with the content of effective components were discovered, so as to provide references for the breeding of new C. nudiflora varieties. Methods Variability analysis was conducted on the content of effective components in 103 C. nudiflora germplasm resources, and 14 SSR markers were used for genetic structure and linkage disequilibrium analysis. Based on this, association analysis between SSR markers and the content of effective components was conducted using the general linear model (GLM) and mixed linear model (MLM) of TASSEL 2.1 software. Results The results showed rich variability in the content of seven effective components in C. nudiflora, making it an ideal material for further association analysis. Population genetic structure analysis indicated that when K＝3, the test materials were divided into three subgroups, each with rich genetic diversity. AMOVA analysis showed that the variation mainly existed within individuals (61%). Among 91 paired SSR loci, both collinear and non-collinear combinations exhibited a certain degree of linkage disequilibrium (LD). Twenty-one paired loci supported by D' statistic probability (P < 0.01) accounted for 23.08% of all loci combinations. D' values mainly ranged between 0.3—0.5 and 0.5—0.7, accounting for 84.62% of the total SSR marker pairs, indicating a lower probability of gene recombination and mutation among the selected germplasm. Association analysis revealed that in the GLM model, 11 markers were significantly or extremely significantly correlated with seven effective component contents (P < 0.05, 0.01). In the MLM model, six markers were significantly or extremely significantly correlated with four effective component contents (P < 0.05, 0.01). In GLM and MLM models, CnH305 was found to be associated with forsythoside B and luteoloside(P < 0.05), CnH155 was associated with luteoloside and acteoside (P < 0.01), and CnH156, CnH357, and CnH205 were associated with isoacteoside. Conclusion The tested C. nudiflora germplasm exhibits rich genetic variation and a certain degree of linkage disequilibrium. Five SSR markers were well associated with the content of effective components, providing a theoretical basis for molecular marker-assisted breeding of C. nudiflora.

R286.12

海南省重大科技计划项目（ZDKJ2021001）;海南省自然科学基金青年基金（322QN393）;海南省重点研发项目（ZDYF2024XDNY216）