目的 建立断血流的HPLC指纹图谱与含量测定方法，结合化学计量学分析断血流的质量。方法 采用HPLC法建立15批断血流药材的指纹图谱，通过对照品比对，指认共有峰，并测定其中4种有效成分（木犀草苷、橙皮苷、槲皮素、醉鱼草皂苷IVb）的含量，使用《中药色谱指纹图谱相似度评价系统（2012版）》、IBM SPSS Statistics 23和Simca软件进行相似度评价、聚类分析（cluster analysis，CA）、主成分分析（principal component analysis，PCA）及正交偏最小二乘判别分析（orthogonal partial least squares discriminant analysis，OPLS-DA），对不同产地断血流的差异进行分析。结果 建立的15批断血流指纹图谱共匹配出21个共有峰，通过与对照品比对，指认出木犀草苷、橙皮苷、迷迭香酸、蒙花苷、槲皮素、醉鱼草皂苷IVb 6个色谱峰，指纹图谱相似度为0.660～1.000；15批次断血流药材中木犀草苷、橙皮苷、槲皮素、醉鱼草皂苷IVb的质量分数分别为0.202 9～2.772 8、4.719 0～16.957 0、0.252 0～1.045 3、0.022 8～0.170 8mg/g；CA分析将样品分为3类；PCA提取出1个主成分；OPLS-DA分析确定木犀草苷、槲皮素2个差异标志物。结论 建立的断血流HPLC指纹图谱与含量测定并结合化学计量学分析方法稳定可靠，可用于断血流药材的质量评价。

Objective To establish a high-performance liquid chromatography (HPLC) fingerprint and content determination method for Duanxueliu (Clinopodii Herba), and to evaluate the quality of Clinopodii Herba through chemometric analysis. Methods HPLC was used to establish fingerprint spectra of 15 batches of Clinopodii Herba, identify common peaks by comparison with reference materials, and determine the content of four effective ingredients (luteolin-7-O-glucoside, hesperidin, quercetin, buddlejasaponins IVb). The similarity evaluation, cluster analysis (CA), principal component analysis (PCA), and orthogonal partial least squares discriminant analysis (OPLS-DA) were performed using traditional Chinese medicine fingerprints (version 2012), IBM SPSS Statistics 23, and Simca software to analyze differences in Clinopodii Herba from different regions of origin. Results A total of 21 common peaks were matched in 15 batches of established Clinopodii Herba fingerprint spectra. Compared with the control sample, six chromatographic peaks were identified, including luteolin-7-O-glucoside, hesperidin, rosmarinic acid, buddleoside, quercetin, buddlejasaponins IVb. The similarity range of the fingerprint spectra was 0.660—1.000; The mass fractions of luteolin-7-O-glucoside, hesperidin, quercetin and buddlejasaponins IVb in 15 batches of Clinopodii Herba were 0.202 9—2.772 8, 4.719 0—16.957 0, 0.252 0—1.045 3, 0.022 8—0.170 8 mg/g, respectively; CA divides the samples into three categories; PCA extracts one principal component; OPLS-DA was used to determine two quality difference biomarkers, luteolin-7-O-glucoside and quercetin. Conclusion HPLC fingerprint and content determination established in this study, combined with chemometric analysis methods, are stable and reliable, and can be used for quality control research of Clinopodii Herba.

R286.2

安徽省重点研究与开发计划：十大皖药断血流产业化关键技术与标准研制（202104h04020036）