目的 研究马鞭草Verbena officinalis醋酸乙酯抗炎活性部位的化学成分。方法 运用多种色谱技术和波谱手段进行分离、纯化和鉴定化合物，通过抑制一氧化氮（NO）生成实验评价新化合物的抗炎活性。结果 从马鞭草醋酸乙酯部位中分离得到8个生物碱类成分，分别为马鞭草碱A（verboffidine A，1）、5-羟基-2-吡啶甲酯（2）、6-羟甲基-3-羟基吡啶（3）、烟酸（4）、尿嘧啶苷（5）、腺苷（6）、苯乙胺（7）和吲哚-3-乙醇（8）。体外抗炎实验显示，化合物1具有抑制NO分泌作用，半数抑制浓度（median inhibition concentration，IC₅₀）为38.5 μmol/L。结论 化合物1为新化合物，命名为马鞭草碱A（verboffidine A），并且具有潜在的抗炎活性；化合物2、3、7和8为首次从马鞭草中分离得到。

Objective To investigate the chemical components of Verbena officinalis of the elthyl acetate fraction with anti-inflammatory activity. Methods The compounds were isolated, purified and identified by various chromatographic techniques and spectral methods. The anti-inflammatory activity of the new compound was evaluated by inhibiting the production of nitric oxide (NO). Results A total of eight alkaloids were isolated from the elthyl acetate fraction of V. officinalis, and their structures were identified as follows: verboffidine A (1), methyl-5-hydroxy-2-pyridinecarboxylate (2), 6-hydroxymethyl-3-pyridinol (3), nicotinic acid (4), uridine (5), adenosine (6), phenethylamine (7) and 2-(1H-indol-3-yl) ethanol (8). In vitro anti-inflammatory activity experiments showed that compound 1 could inhibit NO secretion with a median inhibition concentration (IC₅₀) of 38.5 μmol/L. Conclusion Compound 1 was a new compound, named verboffidine A, and had potential anti-inflammatory activity. Compounds 2, 3, 7 and 8 were isolated for the first time from V. officinalis.

R284.1

国家自然科学基金项目（82160660）；江西省自然科学基金项目（20212ACB206006）；江西中医药大学“1050”工程人才项目（5142001004）；江西中医药大学创新团队（CXTD22015）