目的 研究分心木Diaphragma Juglandis Fructus的化学成分及其体外抗肿瘤活性。方法 综合应用多种色谱技术方法进行分离纯化，结合现代波谱技术及理化性质进行结构鉴定；采用MTT法检测化合物对人子宫颈癌HeLa细胞和人胃癌HGC-27细胞的体外抑制活性。结果 从分心木95%甲醇提取物中分离得到16个化合物，分别鉴定为isosclerone（1）、engelhardione（2）、5-dehydroxy-octahydro-demethoxycurcumin-A（3）、(E)-3,3′-dimethoxy-4,4′-dihydroxystilbene（4）、blumenol A（5）、(＋)-erythro-7-O-ethylguaiacylglycerol（6）、threo-3-(4-hydroxy-3,5-dimethoxyphenyl)-3-ethoxypro-pane-1,2-diol（7）、(6R,9R)-blumenol B（8）、hexahydro-demethoxycurcumin-A（9）、blumenol C（10）、3,4-二羟基苯甲酸乙酯（11）、(＋)-异落叶松脂醇（12）、胡桃宁B（13）、heptanoid（14）、4,17-dimethoxy-2-oxatricyclo [13.2.2.13,7]eicosa-3,5,7(20),15,17,18-hexaene- 10,16-diol（15）、alternariol 9-methyl ether（16）。结论 化合物1～16均为首次从分心木中分离得到，化合物9和13对HeLa细胞具有抑制作用，其半数抑制浓度（median inhibitory concentration，IC₅₀）分别为25.33、16.29 μmol/L，而化合物13对HGC-27细胞表现出一定的活性。

Objective To study the chemical constituents and their antitumor activities from Diaphragma Juglandis Fructus. Methods The 95% methanol extract was isolated and purified by various chromatographic methods, and its structure was identified by modern spectroscopy and physicochemical properties. The in vitro inhibitory activities of the compounds on human cervical cancer cell line Hela and human gastric cancer cell line HGC-27 were detected by MTT method. Results Sixteen compounds were isolated and identified as isosclerone (1), engelhardione (2), 5-dehydroxy-octahydro-demethoxy curcumin-A (3), (E)-3,3′- dimethoxy-4,4′-dihydr-oxystilbene (4), blumenol A (5), (+)-erythro-7-O-ethylguaiacylglycerol (6), threo-3-(4-hydroxy-3,5- dimethoxyphenyl)-3-ethoxypropane-1,2-diol (7), (6R,9R)-blumenol B (8), hexahydro-demethoxycurcumin-A (9)、blumenol C (10), ethyl 3,4-dihydroxybenzoate (11), (+)-isolariciresinol (12), juglanin B (13), heptanoid (14), 4,17-dimethoxy-2-oxatricyclo [13.2.2.13,7] eicosa-3,5,7(20),15,17,18-hexaene-10,16-diol (15) and alternariol 9-methyl ether (16). Conclusion All compounds are isolated from Diaphragma Juglandis Fructus for the first time. Compounds 9 and 13 show inhibitory effect on Hela cells with IC₅₀ of 25.33 and 16.29 μmol/L, respectively. Compound 13 shows certain activity on HGC-27 cells.

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省部共建中亚高发病成因与防治国家重点实验室开放课题资助项目（SKL-HIDCA-2020-ZY12）；上海合作组织科技伙伴计划及国际科技合作计划项目（2020E01011）